FLORAM receives Impact Factor

We are pleased to announce that FLORAM has received its first impact factor rating in the 2022 Journal Citation Reports (JCR).

Now FLORAM has the highest impact factor among Brazilian Forest Sciences journals.

Floresta e Ambiente
Floresta e Ambiente
Original Article Conservation of Nature

Imbibition, Germination and Vigor of Gymnanthes Klotzschiana Müll.Arg. Seeds Under Hypoxia

Émerson Couto da Rocha; Luciana Magda de Oliveira; Vinícius Spolaor Fantinel; Romell Alves Ribeiro Dias

Downloads: 0
Views: 756


Abstract: The objective of this study was to assess hypoxia tolerance in Gymnanthes klotzschiana seeds harvested in two areas (Area 1 - subject to flooding; and Area 2 - not subject to flooding) by the imbibition curve, vigor and germination. Seeds of the two areas were submitted to: one (T-1d), three (T-3d), five (T-5d), ten (T-10d) and twenty days under hypoxia (T-20d); in addition to the control (T-0d). All treatments presented three germination stages. The highest germination was achieved in Area 1 T-1d (90%) and the lowest in Area 2 T-20d (31%). The Area 1 seeds required less time and water content to finish their germination, and they presented greater tolerance to hypoxia; a fact proven by the germination and vigor results. G. klotzschiana seeds harvested in an area subject to flooding are more tolerant to hypoxia and are recommended for restoration projects in this type of area.


environmental stress, submersion, flooding, branquilho


Andrade ER, Melo-Sterza FA, Senada MM, Alfieri, AA. Consequências da produção das espécies reativas de oxigênio na reprodução e principais mecanismos antioxidantes. Revista Brasileira de Reprodução Animal 2010; 34(2): 79-85.

Association of Official Seed Analysts - AOSA. Seed vigour testing handbook. East Lansing; 1983.

Bailey-Serres J, Fukao T, Gibbs DJ, Holdsworth MJ, Lee SC, Licausi F et al. Making sense of low oxygen sensing. Trends in Plant Science 2012; 17(3): 129-138. 10.1016/j.tplants.2011.12.004

Barddal ML. A influência da saturação hídrica na distribuição de oito espécies arbóreas da floresta ombrófila mista aluvial do rio Iguaçu, Paraná, Brasil [dissertation]. Curitiba: Universidade Federal do Paraná; 2006.

Bewley JD, Bradford K, Hilhorst H, Nonogaki H. Physiology of development, germination and dormancy. 3rd ed. New York: Springer; 2013.

Brancalion PHS, Rodrigues RR, Gandolfi S, Kageyama PY, Nave AG, Gandara FB et al. Instrumentos legais podem contribuir para a restauração de florestas tropicais biodiversas. Revista Árvore 2010; 34(3): 455-470. 10.1590/S0100-67622010000300010

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília, DF; 2009.

Carvalho NM, Nakagawa J. Sementes: ciência, tecnologia e produção. 5th ed. Jaboticabal: Funep; 2012.

Colmer TD, Armstrong W, Greenway H, Ismail AM, Kirk GJD, Atwell BJ. Physiological mechanisms of flooding tolerance in rice: transient complete submergence and prolonged standing water. Progress in Botany 2013; 75: 255-307. 10.1007/978-3-642-38797-5_9

Cosmo NL, Nogueira AC, Lima JG, Kuniyoshi YS. Morfologia de fruto, semente e plântula de Sebastiania commersoniana, Euphorbiaceae. Revista Floresta 2010; 40(2): 419-428. 10.5380/rf.v40i2.17837

Crawford RMM, Brandle R. Oxygen deprivation stress in a changing environment. Journal of Experimental Botany 1996; 47(2): 145-159. 10.1093/jxb/47.2.145

Ferreira CS, Piedade MTF, Bonates LC. Germinação de sementes e sobrevivência de plântulas de Himatanthus sucuuba (Spruce) Wood. em resposta ao alagamento, nas várzeas da Amazônia Central. Acta Amazonica 2006; 36(4): 413-418. 10.1590/S0044-59672006000400003

Ferreira CS, Piedade MTF, Franco AC, Gonçalves JFC, Junk WJ. Adaptive strategies to tolerate prolonged flooding in seedlings of floodplain and upland populations of Himatanthus sucuuba, a Central Amazon tree. Aquatic Botany 2009; 90(3): 246-252. 10.1016/j.aquabot.2008.10.006

Ferreira PI, Gomes JP, Batista F, Bernardi AP, Costa NCF, Bortoluzzi RLC et al. Espécies potenciais para recuperação de áreas de preservação permanente no planalto catarinense. Floresta e Ambiente 2013; 20(2):173-182. 10.4322/floram.2013.003

Finch-Savage WE, Leubner-Metzger G. Seed dormancy and the control of germination. New Phytologist 2006; 171(3): 501-523.

Frigerio S, Schenato L, Bossi G, Mantovani M, Marcato G, Pasuto A. Hands-on experience of crowdsourcing for flood risks. An android mobile application tested in Frederikssund, Denmark. International Journal of Environmental Research and Public Health 2018; 15(9): 1-19. 10.3390/ijerph15091926

Gattringer JP, Donath TW, Eckstein RL, Ludewig K, Otte A, Harvolk-Schoning S. Flooding tolerance of four floodplain meadow species depends on age. Plos One 2017; 12(5): 1-15. 10.1371/journal.pone.0176869

Gonçalves JFC, Melo EGF, Silva CEM, Ferreira MJ, Justino GC. Estratégias no uso da energia luminosa por plantas jovens de Genipa spruceana Steyerm submetidas ao alagamento. Acta Botanica Brasilica 2012; 26(2): 391-398. 10.1590/S0102-33062012000200014

Huang X, Schmitt J, Dorn L, Griffith C, Effgen S, Takao S et al. The earliest stages of adaptation in an experimental plant population: strong selection on QTLS for seed dormancy. Molecular Ecology 2010; 19(7): 1335-1351. 10.1111/j.1365-294X.2010.04557.x

Iannone MF, Rosales EP, Groppa MD, Benavides MP. Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf discs exposed to paraquat. Biological Trace Element Research 2012; 146(2): 246-255. 10.1007/s12011-011-9244-1

Instituto Brasileiro de Geografia e Estatística - IBGE. Manual Técnico da Vegetação Brasileira: Sistema fitogeográfico Inventário das formações florestais e campestres Técnicas e manejo de coleções botânicas procedimentos para mapeamentos. Rio de Janeiro; 2012.

Jackson MB, Ram PC. Physiological and molecular basis of susceptibility and tolerance of rice plants to complete submergence. Annals of Botany 2003; 91(2): 227-241.

Jardim Botânico do Rio de Janeiro - JBRJ. Gymnanthes klotzschiana Müll.Arg. Flora do Brasil 2020 [Internet]. [2018?] [cited 2018 Aug. 29]. Available from: Available from: https://bit.ly/2OWgXuW

Kato-Noguchi H. Pyruvate metabolism in rice coleoptiles under anaerobiosis. Plant Growth Regulation 2006; 50(1): 41-46. 10.1007/s10725-006-9124-4

Kirk GJD, Greenway H, Atwell BJ, Ismail AM, Colmer TD. Adaptation of rice to flooded soils. Progress in Botany 2013; 75: 215-253.

Kozlowski TT. Responses of woody plants to flooding and salinity. Tree Physiology Monograph 1997; 7(1): 1-29.

Kraffe E, Marty Y, Guderley H. Changes in mitochondrial oxidative capacities during thermal acclimation of rainbow trout Oncorhynchus mykiss: Roles of membrane proteins, phospholipids and their fatty acid compositions. Journal of Experimental Botany 2007; 210: 149-165.

Li J, Wang Y, Pritchard HW, Wang X. The fluxes of H2O2 and O2 can be used to evaluate seed germination and vigor of Caragana korshinskii. Planta 2014; 239(6): 1363-1373.

López MA, Vicente J, Kulasekaran S, Vellosillo T, Martínez M, Irigoyen ML et al. Antagonistic role of 9-lipoxygenase-derived oxylipins and ethylene in the control of oxidative stress, lipid peroxidation and plant defence. The Plant Journal 2011; 67(3): 447-458. 10.1111/j.1365-313X.2011.04608.x

Luo X, Wu J, Li Y, Nan Z, Guo X, Wang Y et al. Synergistic effects of GhSOD1 and GhCAT1 overexpression in cotton chloroplasts on enhancing tolerance to methyl viologen and salt stresses. Plos One 2013; 8(1): 1-11. 10.1371/journal.pone.0054002

Magneschi L, Perata P. Rice germination and seedling growth in the absence of oxygen. Annals of Botany 2009; 103(2): 181-196. 10.1093/aob/mcn121

Maguire JD. Speed of germination: aid in selection and evaluation for seedling emergence and vigor. Crop Science 1962; 2(2): 176-177.

Marcos-Filho J. Fisiologia de sementes de plantas cultivadas. 2nd ed. Londrina: Abrates; 2015.

Marques MCM, Joly CA. Germinação e crescimento de Calophyllum brasiliense (Clusiaceae), uma espécie típica de florestas inundadas. Acta Botanica Brasilica 2000; 14(1): 113-120. 10.1590/S0102-33062000000100010

Medina CL, Sanches MC, Tucci MLS, Sousa CAF, Cuzzuol GRF, Joly CA. Erythrina speciosa (Leguminosae-Papilionoideae) under soil water saturation: morphophysiological and growth responses. Annals of Botany 2009; 104(4): 671-680.

Narsai R, Whelan J. How unique is the low oxygen response? An analysis of the anaerobic response during germination end comparison with abiotic stress in rice and Arabidopsis. Frontiers in Plant Science 2013; 349(4): 1-14. 10.3389/fpls.2013.00349

Palermo FF, Risso WE, Simonato JD, Martinez CBR. Bioaccumulation of nickel andits biochemical and genotoxic effects on juveniles of the neotropical fish Prochilodus lineatus. Ecotoxicology and Environmental Safety 2015; 116: 19-28. 10.1016/j.ecoenv.2015.02.032

Pisicchio CM, Bianchini E, Pimenta JA, Sert MA, Davanso-Fabro VA, Medri ME. Heliocarpus popayanensis (Malvaceae) tolera a hipóxia do substrato? Acta Scientiarum - Biological Sciences 2010; 32(2): 201-209.

Rodrigues RR, Lima RAF, Gandolfi S, Nave AG. On the restoration of high diversity forests: 30 years of experience in the Brazilian Atlantic Forest. Biological Conservation 2009; 142(6): 1242-1251. 10.1016/j.biocon.2008.12.008

Santos SRG, Aguiar IB. Efeito da temperatura na germinação de sementes de Sebastiania commersoniana (Baillon) Smith & Downs separadas pela coloração do tegumento. Scientia Forestalis 2005; 69: 77-83.

Scarano FR, Pereira TS, Rôças G. Seed germination during floatation and seedling growth of Carapa guianensis, a tree from flood-prone forests of the Amazon. Plant Ecology 2003; 168(2): 291-296. 10.1023/A:1024486715690

Schmiede R, Donath TW, Otte A. Seed bank development after the restoration of alluvial grassland via transfer of seed-containing plant material. Biological Conservation 2009; 142(2): 404-413. 10.1016/j.biocon.2008.11.001

Sevcikova M, Modra H, Slaninova A, Svobodova Z. Metals as a cause of oxidative stress in fish: a review. Veterinarni Medicina 2011; 56(11): 537-546.

Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 2012; 1-26. 10.1155/2012/217037

Silva AC, Higuchi H, van den Berg E, Nunes MH, Carvalho DA. Florestas inundáveis: ecologia, florística e adaptações das espécies. Lavras: Editora UFLA; 2012.

Takahashi H, Saika H, Matsumura H, Nagamura Y, Tsutsumi N, Nishizawa NK et al. Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence. Annals of Botany 2011; 108(2): 253-261. 10.1093/aob/mcr137

Villa-Hernández JM, Dinkova TD, Aguilar-Caballero R, Rivera-Cabrera F, Sánchez de Jiménez E, Pérez-Flores LJ. Regulation of ribosome biogenesis in maize embryonic axes during germination. Biochimie 2013; 95(10): 1871-1879. 10.1016/j.biochi.2013.06.011

Wang WL, Liu SJ, Song SQ, Moller IM. Proteomics of seed development, desiccation tolerance, germination and vigor. Plant Physiology and Biochemistry 2015; 86(1): 1-15. 10.1016/j.plaphy.2014.11.003

Weitbrecht K, Müller K, Leubner-Metzger G. First off the mark: early seed germination. Journal of Experimental Botany 2011; 62(10): 3289-3309. 10.1093/jxb/err030

Xiaolin W, Fen N, Xiuli H, Wei W. Genetic modification for improving seed vigor is transitioning from model plants to crop plants. Frontiers in Plant Science 2017; 8(8): 1-7. 10.3389/fpls.2017.00008

Submitted date:

Accepted date:

5f2850f30e8825ad430e4939 floram Articles
Links & Downloads


Share this page
Page Sections