Floresta e Ambiente
https://floram.org/article/doi/10.1590/2179-8087.022118
Floresta e Ambiente
Original Article Conservation of Nature

Initial in vitro Establishment of the Native Cerrado Orchid Miltonia Flavescens

Camila Soares Rosa Lemes; José Carlos Sorgato; Jackeline Schultz Soares; Danieli Pieretti Nunes; Luan Marlon Ribeiro

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Abstract

Abstract: The situation of native orchids from natural environments becomes more vulnerable each day, especially those not protected by conservation units. Thus, studies on the propagation of species, especially on in vitro germination and initial establishment, are necessary for maintaining species in their habitat and reintroduction in restoration areas. This study verifies the influence of the nutritional composition of the culture media on in vitro germination and initial establishment of Miltonia flavescens. Seeds from natural pollination were sown in four different culture media and put in a growing room under controlled photoperiods and temperature. The germination was evaluated 30 days after sowing, and the initial growth of the seedlings at 120 and 180 days after sowing. The results indicated the Murashige and Skoog medium, complete and with half of the salt concentrations, promoted a higher germination percentage and were more effective in the initial development of M. flavescens seedlings.

Keywords

Orchidaceae, Cerrado, in vitro culture

References

Cardoso JC. Publicação em cultivo in vitro de plantas: qualidade para o avanço científico e tecnológico. Horticultura Brasileira 2014; 32(4): 383-384. 10.1590/S0102-053620140000400002

Freitas EMD, Herrmann MH, Bruisma G, Périco E, Araújo AG. Propagação in vitro de Cattleya intermedia Graham ex Hook (Orchidaceae) em diferentes meios de cultura. Caderno Pedagógico 2014; 11(1): 30-41.

Gamborg OL. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiology 1970; 45(4): 372-375. 10.1104/pp.45.4.372

Gale SW, Fischer GA, Cribb PJ, Fay MF. Orchid conservation: bridging the gap between science and practice. Botanical Journal of the Linnean Society 2018; 186(4): 425-434. 10.1093/botlinnean/boy003

Jardim Botânico do Rio de Janeiro - JBRJ. Orchidaceae [Internet]. [2018?] [cited 2018 Aug. 6]. Avaliable from: Avaliable from: https://bit.ly/3a9lxza

Lam Y, Ng TB, Yao RM, Shi J, Xu K, Sze SCW, Zhang KY. Evaluation of chemical constituents and important mechanism of pharmacological biology in Dendrobium plants. Evidence-Based Complementary and Alternative Medicine 2015; 2015(1): 1-25. 10.1155/2015/841752

Lo SH, Nalawade SM, Kuo CL, Chen CL, Tsay HS. Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense Makino: a medicinally important orchid. In Vitro Cellular & Development Biology-Plant 2004; 40(5): 528-535. 10.1079/IVP2004571

Martini PC, Willadino L, Alves GD, Donato VMTS. Propagação da orquídea Gongora quinquenervis por semeadura in vitro. Pesquisa Agropecuária Brasileira 2001; 36(10): 1319-1324. 10.1590/S0100-204X2001001000015

Muller TS, Dewes D, Karsten J, Schuelter AR, Stefanello S. Crescimento in vitro e aclimatação de plântulas de Miltonia flavescens. Revista Brasileira de Biociências 2007; 5(2): 252-254.

Nadarajan J, Wood S, Marks TR, Seaton PT, Pritchard HW. Nutritional requirements for in vitro seed germination of 12 terrestrial, lithophytic and epiphytic orchids. Journal of Tropical Forest Science 2011; 23(2): 204-212.

Porte LF, Santin SMO, Chiavelli LUR, Silva CC, Faria TJ, Faria RT, Ruiz AL, Carvalho JE, Pomini AM. Bioguided identification of antifungal and antiproliferative compounds from the Brazilian orchid Miltonia flavescens Lindl. Zeitschrift fur Naturforschung C 2014; 69(1-2): 46-52. 10.5560/znc.2012-0192

Ramalho AMZ, Pimenta HCD. Valoração econômica do dano ambiental ocasionado pela extração ilegal da orquídea Cattleya granulosa no Parque Natural Dom Nivaldo Monte, Natal/RN. Holos 2010; 26(1): 62-82. 10.15628/holos.2010.333

Schneiders D, Pescador R, Booz MR, Suzuki RM. Germinação, crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp., Orchidaceae). Revista Ceres 2012; 59(2): 185-191. 10.1590/S0034-737X2012000200006

Silva JAT, Tsavkelova EA, Ng TB, Parthibhan S, Dobránszki J, Cardoso JC, Rao MV et al. Asymbiotic in vitro seed propagation of Dendrobium. Plant Cell Reports 2015; 34(10): 1685-1706. 10.1007/s00299-015-1829-2

Silva CS, Araújo LG, Sousa KCI, Silva DM, Sibov ST, Faria PR. Germinação e desenvolvimento in vitro de orquídea epífita do Cerrado. Ornamental Horticulture 2017; 23(1): 96-100.

Soares JS, Rosa YBCJ, Tatara MB, Sorgato JC, Lemes CSR. Identificação da viabilidade de sementes de orquídeas pelo teste de tetrazólio. Semina: Ciências Agrárias 2014; 35(5): 2275-2284. 10.5433/1679-0359.2014v35n5p2275

Stewart J. Orchid propagation by tissue culture techniques - past, present and future. In: Pritchard HW, editor. Modern methods in orchid conservation: the role of physiology, ecology and management. Cambridge: Cambridge University Press; 1989. p. 87-100.

Suzuki RM, Almeida V, Pescador R, Ferreira WM. Germinação e crescimento in vitro de Cattleya bicolor Lindley (Orchidaceae). Hoehnea 2010; 37(4): 731-742. 10.1590/S2236-89062010000400004

Suzuki RM, Moreira VC, Nakabashi M, Ferreira WM. Estudo da germinação e crescimento in vitro de Hadrolaelia tenebrosa (Rolfe) Chiron & V. P. Castro (Orchidaceae), uma espécie da flora brasileira ameaçada de extinção. Hoehnea 2009; 36(4): 657-666. 10.1590/S2236-89062009000400006

Tavares AR, Young JLM, Ori SS, Kanashiro S, Lima GPP, Chu EP, Suzuki RM. Orchid in vitro growth as affected by nitrogen levels in the culture medium. Horticultura Brasileira 2012; 30(1): 119-124. 10.1590/S0102-05362012000100020

Tsering J, Tam N, Tag H, Gogoi BJ, Apang O. Medicinal orchids of Arunachal Pradesh: a review. Bulletin of Arunachal Forest Research 2017; 32(1-2): 1-16.

Vudala SM, Ribas LLF. Seed storage and asymbiotic germination of Hadrolaelia grandis (Orchidaceae). South African Journal of Botany 2017; 108(1): 1-7. 10.1016/j.sajb.2016.09.008
 


Submitted date:
05/07/2018

Accepted date:
11/05/2018

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