Floresta e Ambiente
https://floram.org/article/doi/10.1590/2179-8087-FLORAM-2022-0078
Floresta e Ambiente
Original Article Silviculture

Mobilization of Storage Reserves in Dalbergia spruceana Benth. (Fabaceae) Seeds During Germination at Different Temperatures

Clenes Cunha Lima, Eduardo Euclydes Lima Borge, Ely Simone Cajueiro Gurgel

Downloads: 0
Views: 231

Abstract

Temperature may affect the mobilization and hydrolysis of storage reserves for energy production during seed germination. This study investigated germination performance and reserve mobilization in Dalbergia spruceana Benth. seeds incubation at 20, 25, 30, 35, and 40°C. The germination process was favored by incubation at 25 to 35°C and negatively affected at 20 and 40°C. At 35°C there reduction in germination speed, however, without significantly compromising the final germination percentage. The results showed that lipids and proteins are the predominant metabolites in D. spruceana seeds. Mobilization of soluble sugars was highest at 25 and 30°C. At 20 and 40°C, mobilization occurred more slowly, negatively affecting germination. This finding, combined with changes in lipid and protein reserves, suggests that lipid and protein hydrolysis products were used for starch synthesis. Reserve mobilization patterns in D. spruceana embryos were influenced by germination temperature, with the highest utilization efficiency occurring between 25 and 35°C.

Keywords

Amazon; Amazon rosewood; biomolecules; cell metabolism; heat stress

References

  • Alencar NLM, Innecco R, Gomes-Filho E, Gallão MI, Alvarez-Pizarro JC, Prisco JT, Oliveira AB. Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae). Anais da Academia Brasileira de Ciências 2012; 84(3): 823-832.

  • Ataíde GM, Borges EEL, Picoli EAT, Filho ATL, Flores AV. Alterações nas reservas de sementes de Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) durante a germinação em diferentes temperaturas. Revista Brasileira de Ciências Agrárias 2017; 12(3): 372-379.

  • Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H. Seeds: physiology of development, germination and dormancy. 3rd ed. New York: Springer; 2013.

  • Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília: SDA/ACS; 2009.

  • Buckeridge MS, Dietrich SMC. Galactomanans from Brazilian legume seeds. Revista Brasileira de Botânica 1990; 13(1): 109-112.

  • Carvalho AMA. Synopsis of the genus Dalbergia (Fabaceae: Dalbergieae) in Brazil. Brittonia 1997; 49(1): 87-109.

  • Cunniff P. Official methods of analysis of Aoac International. 16ª ed., AOAC; 1995.

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Annalitical Chemistry 1956; 28(1): 350-356.

  • Duncan C, Schultz N, Lewandrowski, W, Good, MK, Cook S. Lower dormancy with rapid germination is an important strategy for seeds in an arid zone with unpredictable rainfall. PLoS One 2019; 14(9): 1-19.

  • Felix FC, Medeiros JAD, Ferrari CS, Pacheco MV, Torres SB. Molecular aspects during seed germination of Erythrina velutina Willd. under different temperatures (Part 1): reserve mobilization. Journal of Seed Science 2020a; 42(1): 1-10.

  • Felix FC, Medeiros JAD, Ferrari CS, Pacheco MV, Torres SB. Molecular aspects during seed germination of Erythrina velutina Willd. under different temperatures (Part 2): isoenzyme activity and DNA integrity. Journal of Seed Science 2020b, 42(1): 1-19.

  • Gama JRV, Pinheiro JC. Inventário florestal para adequação ambiental da fazenda Santa Rita, município de Santarém, Estado do Pará. Floresta 2010; 40(3): 585 - 592.

  • Heldt, HW. Plant Biochemistry, Elsevier, San Diego; 2005.

  • Lopes LS, Gallao MI, Bertini CHCM. Mobilização de reservas durante a germinação de sementes de Jatropha. Revista Ciência Agronômica 2013; 44(2): 371-378.

  • Maguire JD. Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Science 1962; 2(2): 176-77.

  • Mazzottini-Dos-Santos HC, Ribeiro LM, Oliveira DMT. Roles of the haustorium and endosperm during the development of seedlings of Acrocomia aculeata (Arecaceae): dynamics of reserve mobilization and accumulation. Protoplasma 2017; 254 (1): 1563-1578.

  • Nievola CC, Carvalho CP, Carvalho V, Rodrigues E. Rapid responses of plants to temperature changes. Temperature 2017; 4 (4): 371-405.

  • Oliveira LA, De Souza GA, Silva ST, Rocha AAG, Picoli EAT, Pereira DS, Donzeles SML, Ribeiro MF, Ferreira WPM. Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds. Coffee Science 2020; 15(1): 1-14.

  • Passos LP. Métodos analíticos e laboratoriais em fisiologia vegetal. Coronel Pacheco: Embrapa-CNPGL; 1996.

  • Paula SO, Sousa JA, Brito ES, Gallão MI. The morphological characterization of the dry seeds and reserve mobilization during germination in Morinda citrifolia L. Revista Ciência Agronômica 2016; 47(3): 556-563.

  • Reis LP, Borges EEL, Souza GA, Brito DS. Relationships between substrate and the mobilization of reserve with temperature during seed germination of Ormosia coarctata Jack. Journal of Seed Science 2020; 42(1).

  • Santos MM, Doriguetto IR, Borges EEL, Andrade GM. Germinação e mobilização de reservas em sementes de amendoim-bravo (Pterogyne nitens) sob estresse térmico. Revista Brasileira de Biociências 2019; 17(1): 32-37.

  • Santos MM, Borges EEL, Ataíde GM, Pires RMO, Rocha DK. Enzyme activity in the micropylar region of Melanoxylon brauna Schott seeds during germination under heat stress conditions. Journal of Seed Science 2020; 42(1): 1:10.

  • Silva DJ. Análise de alimentos - métodos químicos e biológicos. Viçosa, MG: Universidade Federal de Viçosa; 1990.

  • Souza LAG. Guia da biodiversidade de Fabaceae do Alto Rio Negro. 2012. 118 p. Disponível em: Disponível em: https://www.inpa.gov.br/arquivos/livro_fronteiras/Pesquisadores/10-Luiz-Augusto/Guia%20Fabaceae%20-%20Livro.pdf Acesso em: 27 de abril de 2020.
    » https://www.inpa.gov.br/arquivos/livro_fronteiras/Pesquisadores/10-Luiz-Augusto/Guia%20Fabaceae%20-%20Livro.pdf

  • Tesfay SZ, Modi AT, Mohammed F. The effect of temperature in moringa seed phytochemical compounds and carbohydrate mobilization. South African Journal of Botany 2016; 102(1): 190-196.

  • Tozzi HH, Takaki M. Histochemical analysis of seed reserve mobilization in Passiflora edulis Sims fo. flavicarpa O. Deg. (yellow passion fruit) during germination. Brazilian Journal Biology 2011; 71(3): 701-708.

  • Walters DM, Arendt EK, Moroni AV. Visão geral sobre os mecanismos de germinação e fermentação e seu significado para café e café qualidade da bebida. Avaliações críticas em ciência alimentar e Nutrition 2017; 57(2): 259-274.

  • Xian M, Luo T, Khan MN, Hu L, Xu Z. Identifying differentially expressed genes associated with tolerance against low temperature stress in Brassica napus through transcriptome analysis. International Journal Of Agriculture & Biology 2017; 16(2): 273-281.

  • Yang N, Guo X, Wu Y, Hu X, Ma Y, Zhang YE, Wang H, Tang Z. The inhibited seed germination by ABA and MeJA is associated with the disturbance of reserve utilizations in Astragalus membranaceus. Journal of Plant Interactions 2018; 13(1): 388-397.

  • Zhao M, Zhang H, Yan H, Qiu L, Baskin CC. Mobilization and role of starch, protein, and fat reserves during seed germination of six wild grassland species. Frontier in Plant Science 2018; 27(1).


Submitted date:
10/21/2022

Accepted date:
06/28/2023

65009866a9539556b03bac84 floram Articles

FLORAM

Share this page
Page Sections