Floresta e Ambiente
https://floram.org/article/doi/10.1590/2179-8087-FLORAM-2020-0022
Floresta e Ambiente
Original Article Silviculture

Physiological Quality of Stored Dipteryx alata Vogel Seeds: Tetrazolium Test and Radiographic Imaging Standardization

Glicélia Pereira Silva, Juliana de Fátima Sales, Jacson Zuchi, Kelly Juliane Telles Nascimento, Lílian Abadia da Silva, Moara Mariely Vinhais Souza, Arthur Almeida Rodrigues

Downloads: 0
Views: 886

Abstract

The baru (Dipteryx alata Vogel), is fruit tree displaying economic, environmental, industrial and food importance. The objective of this study was to adapt to the tetrazolium test methodology to baru seeds and evaluate the physiological quality of seeds stored for 12 months at 10 and 20 ºC, using X-ray images as a toll for detection damage throughout the storage period while also carrying out germination, emergence and electrical conductivity tests. Baru seeds remained viable for 12 months of storage, albeit with a small decrease in vigor after nine months. The applied tetrazolium test methodology was efficient in determining internal seed damage. The pre-soak time for the tetrazolium test and solution concentration was 24 hours and 0.05%. No changes in seed morphology and radiographic images were observed during the storage periods, regardless of the assessed temperature. The X-ray test enabled a precise visualization of internal baru seed structures.

Keywords

Storage; baru; Viability; X-ray; vigor

References

  • Abreu LAS, Carvalho LM, Pinto CAG, Kataoka VY. Teste de condutividade elétrica na avaliação de sementes de girassol armazenadas sob diferentes temperaturas. Revista Brasileira de Sementes 2011; 33(4):593-601. http://dx.doi.org/10.1590/S0101-31222011000400005.
    » https://doi.org/http://dx.doi.org/10.1590/S0101-31222011000400005

  • Abreu LAS, Carvalho MLM, Pinto CAG, Kataoka VY, Silva, TTA. Deterioration of sunflower seeds during storage. Journal of Seed Science 2013; 35(2):240-247. https://doi.org/10.1590/S2317-15372013000200015.
    » https://doi.org/https://doi.org/10.1590/S2317-15372013000200015

  • Afzal I, Jaffar I, Zahid S, Rehman HUr, Barsa SMA. Physiological and biochemical changes during hermetic storage of Moringa oleifera seeds. Shout. South African Journal of Botany 2019; 129: 435-441. https://doi.org/10.1016/j.sajb.2019.11.011.
    » https://doi.org/https://doi.org/10.1016/j.sajb.2019.11.011

  • Alves MVP. Armazenamento, viabilidade e emergência de sementes e diásporos de Emmotum nitens (Benth) Miers. Revista Verde de Agroecologia e Desenvolvimento Sustentável 2012; 7:163-168.

  • Araújo MS, Ferraz M, Américo FKA, Silva FS, Dantas BF, Cruz CRP. Seed quality of Amburana cearensis (Allemão) A.C. Sm. (Fabaceae) is influenced by storage condition. Journal of Seed Science 2017; 39(4):401-409. http://dx.doi.org/10.1590/2317-1545v39n4179328.
    » https://doi.org/http://dx.doi.org/10.1590/2317-1545v39n4179328

  • Arruda N, Cicero SM, Junior FGG. Radiographic analysis to assess the seed structure of Crotalaria juncea L. Journal of Seed Science 2016; 1-8. http://dx.doi.org/10.1590/2317-1545v38n2155116.
    » https://doi.org/http://dx.doi.org/10.1590/2317-1545v38n2155116

  • Ataíde GD, Flores AV, Borges EEL. Alterações fisiológicas e bioquímicas em sementes de Pterogyne nitens Tull. Durante o envelhecimento artificial. Pesquisa Agropecuária Tropical 2012; 42:71-76. http://dx.doi.org/10.1590/S1983-40632012000100010.
    » https://doi.org/http://dx.doi.org/10.1590/S1983-40632012000100010

  • Azerêdo GA, Paula RC, Valeri SV. Viabilidade de sementes de Piptadenia moniliformis Benth. pelo teste de tetrazólio. Revista Brasileira de Sementes 2011; 33:61-68. http://dx.doi.org/10.1590/S0101-31222011000100007.
    » https://doi.org/http://dx.doi.org/10.1590/S0101-31222011000100007

  • Bragante RB, Hell AF, Silva, JPN, Caetano DC, Ribeiro, RCLF, Barbedo CJ. Physiological and metabolic responses of immature and mature seeds of Libidibia ferrea ((Mart. ex Tul.) L.P Queiroz) under contrasting storage temperatures. Brazillian Journal of Botany 2018; 41(1):43-55. https://doi.org/10.1007/s40415-018-0442-3.
    » https://doi.org/https://doi.org/10.1007/s40415-018-0442-3

  • Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. 2009. Brasília, DF: Mapa/ACS. 395p.

  • Brannstrom C, Jepson W, Filippi AM, Redo D, Xu Z, Ganessh S. Land change in the Brazilian savanna (Cerrado), 1986-2002: comparative analysis and implications for land-use policy. Land Use Policy 2008; 25:579-595. https://doi.org/10.1016/j.landusepol.2007.11.008.
    » https://doi.org/https://doi.org/10.1016/j.landusepol.2007.11.008

  • Brito WAL, Pereira KTO, Nogueira NW, Torres SB, Paiva EP. Evaluation of viability of Tabebuia aurea seeds through tetrazolium test. Revista Caatinga 2020; 33(4):993-999. http://dx.doi.org/10.1590/1983-21252020v33n414rc.
    » https://doi.org/http://dx.doi.org/10.1590/1983-21252020v33n414rc

  • Burg WJ, Van Der, Aartese JW, Zwol RAV, Jalink H, Bino RJ. Predicting tomato seedling morphology by x-ray analysis of seeds. Journal of the American Society for Horticultural Science 1994; 119:258-263. https://doi.org/10.21273/JASHS.119.2.258.
    » https://doi.org/https://doi.org/10.21273/JASHS.119.2.258

  • Carvalho FMV, Júnior PDM, Ferreira LG. The Cerrado into-pieces: Habitat fragmentation as a function of landscape use in the savannas of central Brazil. Biological Conservation 2009; 142:1392-1403. https://doi.org/10.1016/j.biocon.2009.01.031.
    » https://doi.org/https://doi.org/10.1016/j.biocon.2009.01.031

  • Carvalho NM, Nakagawa J. Sementes: ciência, tecnologia e produção. 4.ed. Jaboticabal: 2000; FUNEP, 588p.

  • Carvalho NM, Nakagawa J. Sementes: ciências, tecnologias e produção. 5.ed., Jaboticabal; 2012; FUNEP, p. 590.

  • Carvalho SMC, Torres SB, Benedito CP, Nogueira NW, Souza AAT, Neta MLS. Viability of Libidibia ferrea ((Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test. Journal of Seed Science 2017; 39(1):7-12. http://dx.doi.org/10.1590/2317-1545v39n1163784.
    » https://doi.org/http://dx.doi.org/10.1590/2317-1545v39n1163784

  • Cícero MS, Junior HLB. Avaliação do relacionamento entre danos mecânicos e vigor, em sementes de milho, por meio da análise de imagens. Revista Brasileira de Sementes 2003; 25:29-36. https://doi.org/10.1590/S0101-31222003000100006.
    » https://doi.org/https://doi.org/10.1590/S0101-31222003000100006

  • Fernandes DC, Freitas JB, Czedera LP, Navesb MMV. Nutricional composition and protein value of the baru (Dipteryx alata Vog.) almond from the Brazilian Savana. Journal of the Science of Food and Agriculture 2010; 90:1650-1655. https://doi.org/10.1002/jsfa.3997.
    » https://doi.org/https://doi.org/10.1002/jsfa.3997

  • Fessel SA, Panobianco M, Souza CR, Vieira RD. Teste de condutividade elétrica em sementes de soja armazenadas sob diferentes temperaturas. Bragantia 2010; 69:207-214. http://dx.doi.org/10.1590/S0006-87052010000100026.
    » https://doi.org/http://dx.doi.org/10.1590/S0006-87052010000100026

  • França Neto JB, Krzyzanowski FC, Costa NP. O teste de tetrazólio em sementes de soja. Londrina: EMBRAPA-CNPSo, 72p. (EMBRAPA-CNPSo, 1998; Documentos, 116).

  • Freire JM, Rouws JRC, Breier TB, Ataídes GM. Dying and storage of Melanoxylon braúna Schott. seeds. Brazilian Journal of Biology 2021; 81(2):464-473. https://doi.org/10.1590/1519-6984.232578.
    » https://doi.org/https://doi.org/10.1590/1519-6984.232578

  • Freitas DA, Durães AFS, Firmo, DHT, Pinho NB, Carvalho LR. Levantamento de dados de espécies florestais nativas do Cerrado: um meio para bancos de sementes implantados que permitem restauração de ecossistemas florestais. Brazilian Journal of Animal and Environmental Research 2019; 2(5):1569-1583.

  • Gomes KBP, Matos JMM, Martins IS, Martins RC. X-ray test to evaluate the physiological potencial of Platypodium elegans Vog. Seeds (Fabaceae). Scientia Agropecuária 2016; 7(3):305-311. DOI: 10.17268/sci.agropecu.2016.03.19.
    » https://doi.org/10.17268/sci.agropecu.2016.03.19

  • ISTA. International Seed Testing Association. International Rules for Seed Testing. Zurich, 2004, p. 174.

  • Klink CA, Machado R. Conservation of the Brazilian Cerrado. Conservation Biology 2005; 19:707-713. https://doi.org/10.1111/j.1523-1739.2005.00702.x.
    » https://doi.org/https://doi.org/10.1111/j.1523-1739.2005.00702.x

  • Kohama S, Maluf AM, Bilia DAC, Barbedo CJ. Secagem e armazenamento de sementes de Eugenia brasiliense LAM. (Grumixameira). Revista Brasileira de Sementes 2006; 28:72-78. http://dx.doi.org/10.1590/S0101-31222006000100010.
    » https://doi.org/http://dx.doi.org/10.1590/S0101-31222006000100010

  • Lima LKS, Dutra AS, Santos CS, Barros GL. Agropecuária científica no semiárido 2013; 9:01-06.

  • Machado CF, Cicero SM. Aroeira-branca [Lithraea molleoides (Vell.) Engl. - Anacardiaceae] seed quality evaluation by the x-ray test. Scientia Agricola 2003; 60:393-397. http://dx.doi.org/10.1590/S0103-90162003000200026.
    » https://doi.org/http://dx.doi.org/10.1590/S0103-90162003000200026

  • Medeiros AD, Araújo JO, León MJ, Silva LJ, Dias DCFS. Parameters based on X-ray images to assess the physical and physiological quality of Leucaena leucocephala seeds. Ciência e Agrotecnologia 2018; 42(6):643-652. http://dx.doi.org/10.1590/1413-70542018426023318.
    » https://doi.org/http://dx.doi.org/10.1590/1413-70542018426023318

  • Medeiros JAD, Nunes SP, Félix FC, Ferrari CS, Pacheco MV, Torres SB. Vigor test of (strong) normal intact Amburana cearenses (Allemão) A.C. Smith seedlings. Journal of Seed Science 2020; 42:1-10. https://doi.org/10.1590/2317-1545v42221611.
    » https://doi.org/https://doi.org/10.1590/2317-1545v42221611

  • Mota LHS, Scalon SPQ, Heinz R. Sombreamento na emergência de plântulas e no crescimento inicial de Dipteryx alata Vog. Ciência Florestal 2012; 22:423-431. http://dx.doi.org/10.5902/198050986611.
    » https://doi.org/http://dx.doi.org/10.5902/198050986611

  • Murthy UMN, Kumar PP, Sun WQ. Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczeck: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany 2003; 54:1057-106. https://doi.org/10.1093/jxb/erg092.
    » https://doi.org/https://doi.org/10.1093/jxb/erg092

  • Nagel M, Börner A. The longevity of crop seeds stored under ambient conditions. Seed Science Research 2010; 20:1-12. http://dx.doi.org/10.1017/S0960258509990213.
    » https://doi.org/http://dx.doi.org/10.1017/S0960258509990213

  • Oliveira AN, Silva AC, Rosado SCS, Rodrigues EAC. Variações genéticas para características do sistema radicular de mudas de baru (Dipteryx alata Vog.). Revista Árvore 2006; 30:905-909. http://dx.doi.org/10.1590/S0100-67622006000600005.
    » https://doi.org/http://dx.doi.org/10.1590/S0100-67622006000600005

  • Oliveira LM, Carvalho MLM, Davide AC. Utilização do teste de raios-x na avaliação da qualidade de sementes de canafístula (Peltophorum dubium (Sprengel) Taubert. Revista Brasileira de Sementes 2003; 25:116-120. http://dx.doi.org/10.1590/S0101-31222003000100018.
    » https://doi.org/http://dx.doi.org/10.1590/S0101-31222003000100018

  • Panobianco M, Vieira RD, Perecin D. Electrical conductivity as an indicator of pea seed aging of stored at different temperatures. Scientia Agricola 2007; 64:119-124. http://dx.doi.org/10.1590/S0103-90162007000200003.
    » https://doi.org/http://dx.doi.org/10.1590/S0103-90162007000200003

  • Pinto TLF, Mondo VV, Gomes-Júnior FG, Cícero SM. Análise de imagem na avaliação de danos mecânicos em soja. Pesquisa Agropecuária Tropical 2012; 42:310-316. http://dx.doi.org/10.1590/S1983-40632012000300010.
    » https://doi.org/http://dx.doi.org/10.1590/S1983-40632012000300010

  • Pinto TLF, Cicero MS, Forti VA. Avaliação de danos por umidade, em sementes de soja, utilizando a técnica da análise de imagens. Revista Brasileira de Sementes 2007; 29:28-34. http://dx.doi.org/10.1590/S0101-31222007000300004.
    » https://doi.org/http://dx.doi.org/10.1590/S0101-31222007000300004

  • Pontes CA, Corte VB, Borges EEL, Silva AG, Borges RCG. Influência da temperatura de armazenamento na qualidade das sementes de Caesalpinia peltophoroides Benth. (Sibipiruna). Revista Árvore 2006; 30:43-48. http://dx.doi.org/10.1590/S0100-67622006000100006.
    » https://doi.org/http://dx.doi.org/10.1590/S0100-67622006000100006

  • Pupim TL, Novembre ADLC, Carvalho MLM, Cicero SM. Adequação do teste de raio x para avaliação da qualidade de sementes de embaúba (Cecropia pachystachya Trec.). Revista Brasileira de Sementes 2008; 30(2):028-032.

  • Ribeiro RC, Gomes SEV, Dantas BF. Physiological quality of Erythrina velutina Willd. seeds (FABACEAE) under different storage conditions. Scientia Forestalis, 2018; 46(120):562-570. DOI: dx.doi.org/10.18671/scifor.v46n120.05.
    » https://doi.org/dx.doi.org/10.18671/scifor.v46n120.05

  • Silva LJ, Dias DCFS, Sekita MC, Finger FL. Lipid peroxidation and antioxidant enzymes of Jatropha curcas L. seeds stored at different maturity stages. Acta Scientiarum Agronomy 2018; 40:1-10. Doi: 10.4025/actasciagron.v40i1.34978.
    » https://doi.org/10.4025/actasciagron.v40i1.34978

  • Silva GP, Sales JF, Nascimento KJT, Rodrigues AA, Camelo GN, Borges EEDL. Biochemical and physiological changes in Dipteryx alata Vog. seeds during germination and accelerated aging. South African Journal of Botany 2020; 131:84-92. https://doi.org/10.1016/j.sajb.2020.02.007
    » https://doi.org/https://doi.org/10.1016/j.sajb.2020.02.007

  • Smaniotto TA, Resende O, Marçal KAF, Oliveira DEC, Simon GA. Qualidade fisiológica das sementes de soja armazenadas em diferentes condições. Revista Brasileira de Engenharia Agrícola e Ambiental 2014; 18:443-453. http://dx.doi.org/10.1590/S1415-43662014000400013.
    » https://doi.org/http://dx.doi.org/10.1590/S1415-43662014000400013

  • Soares TN, Chaves LJ, Telles MPC, Diniz-Filho JAF, Resende LV. Landscape conservation genetics of Dipteryx alata Vog. (“baru tree”: Fabaceae) from Cerrado region of central Brazil. Genética 2008; 9-19 http://dx.doi 10.1007/s10709-007-9144-7.
    » https://doi.org/http://dx.doi 10.1007/s10709-007-9144-7

  • Toledo MZ, Fonseca NR, César ML, Soratto RP, Cavariani C, Crusciol CAC. Qualidade fisiológica e armazenamento de sementes de feijão em função da aplicação tardia de nitrogênio em cobertura. Pesquisa Agropecuparia Tropical 2009; 39:124-133.

  • Vieira RD, Krzyzanowski FC. Teste de Condutividade Elétrica. In: Krzyzanowsky FC, Vieira RD, França-Neto JB. Vigor de Sementes: Conceitos e Testes. 1999; Londrina: ABRATES, p.4/1-4/26.

  • Walters C, Wheeler LM, Grotenhuins JM. Longevity of seeds stored in a genebank: species characteristics. Seed Science Research 2005; 15:1-20. https://doi.org/10.1079/SSR2004195.
    » https://doi.org/https://doi.org/10.1079/SSR2004195

  • Walter LS, Gabira MM, Silva MA, Nogueira AC, Kratz D. Adjustments in the tetrazolium test methodology for assessing the physiological quality of Jatropha mollissima (Euphorbiaceae). Bosque 2020; 41(1):77-88. DOI: 10.4067/S0717-92002020000100077.
    » https://doi.org/10.4067/S0717-92002020000100077


Submitted date:
04/15/2020

Accepted date:
06/30/2021

610d993aa9539507de368eb3 floram Articles

FLORAM

Share this page
Page Sections